nf κb dna binding analysis total proteins Search Results


s. aureus topoisomerase iv  (TopoGEN Inc)
90
TopoGEN Inc s. aureus topoisomerase iv
Inhibitory effects of gemifloxacin and ciprofloxacin on wild-type and mutant ( grlA -deficient) <t> topoisomerase </t> IV and wild-type gyrase
S. Aureus Topoisomerase Iv, supplied by TopoGEN Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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p68-cdna (nucleotide sequence 1–1849; 39)  (Biozol Diagnostica Vertrieb GmbH)
90
Biozol Diagnostica Vertrieb GmbH p68-cdna (nucleotide sequence 1–1849; 39)
Inhibitory effects of gemifloxacin and ciprofloxacin on wild-type and mutant ( grlA -deficient) <t> topoisomerase </t> IV and wild-type gyrase
P68 Cdna (Nucleotide Sequence 1–1849; 39), supplied by Biozol Diagnostica Vertrieb GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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zinc finger protein 91  (Kaltenbach GmbH)
90
Kaltenbach GmbH zinc finger protein 91
Inhibitory effects of gemifloxacin and ciprofloxacin on wild-type and mutant ( grlA -deficient) <t> topoisomerase </t> IV and wild-type gyrase
Zinc Finger Protein 91, supplied by Kaltenbach GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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vegf-b reporter construct containing the 5′ flanking region (−851 to +156) of human vegf-b  (SwitchGear Genomics)
90
SwitchGear Genomics vegf-b reporter construct containing the 5′ flanking region (−851 to +156) of human vegf-b
Inhibitory effects of gemifloxacin and ciprofloxacin on wild-type and mutant ( grlA -deficient) <t> topoisomerase </t> IV and wild-type gyrase
Vegf B Reporter Construct Containing The 5′ Flanking Region (−851 To +156) Of Human Vegf B, supplied by SwitchGear Genomics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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vegf-b reporter construct containing the 5′ flanking region (−851 to +156) of human vegf-b - by Bioz Stars, 2026-10
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dna topoisomerase iiα (top iia)  (BD Diagnostics)
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BD Diagnostics dna topoisomerase iiα (top iia)
Inhibitory effects of gemifloxacin and ciprofloxacin on wild-type and mutant ( grlA -deficient) <t> topoisomerase </t> IV and wild-type gyrase
Dna Topoisomerase Iiα (Top Iia), supplied by BD Diagnostics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human topoisomerase ii alpha (topoiiα) promoter  (Promega)
90
Promega human topoisomerase ii alpha (topoiiα) promoter
Infrared gel electrophoresis images of DNase I footprinting products following incubation of compounds 1–6 with <t>TOPOIIα</t> promoter DNA. Compounds were incubated for 17 h with TOPOIIα promoter DNA IR-labelled on the sense (A) or antisense (B) strand. Each gel consists of 50 lanes: two GA marker lanes (GA) and six sets of eight lanes for compound (l–r; 0, 0.0016, 0.008, 0.04, 0.2, 1, 5 and 25 μM). The sequence positions relating to Supplementary Figure 1 are given vertically.
Human Topoisomerase Ii Alpha (Topoiiα) Promoter, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mt2-mmp plasmid dna  (Johns Hopkins HealthCare)
90
Johns Hopkins HealthCare mt2-mmp plasmid dna
Infrared gel electrophoresis images of DNase I footprinting products following incubation of compounds 1–6 with <t>TOPOIIα</t> promoter DNA. Compounds were incubated for 17 h with TOPOIIα promoter DNA IR-labelled on the sense (A) or antisense (B) strand. Each gel consists of 50 lanes: two GA marker lanes (GA) and six sets of eight lanes for compound (l–r; 0, 0.0016, 0.008, 0.04, 0.2, 1, 5 and 25 μM). The sequence positions relating to Supplementary Figure 1 are given vertically.
Mt2 Mmp Plasmid Dna, supplied by Johns Hopkins HealthCare, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pt -gfp cdna  (NanoLight Inc)
90
NanoLight Inc pt -gfp cdna
Infrared gel electrophoresis images of DNase I footprinting products following incubation of compounds 1–6 with <t>TOPOIIα</t> promoter DNA. Compounds were incubated for 17 h with TOPOIIα promoter DNA IR-labelled on the sense (A) or antisense (B) strand. Each gel consists of 50 lanes: two GA marker lanes (GA) and six sets of eight lanes for compound (l–r; 0, 0.0016, 0.008, 0.04, 0.2, 1, 5 and 25 μM). The sequence positions relating to Supplementary Figure 1 are given vertically.
Pt Gfp Cdna, supplied by NanoLight Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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recombinant proteins phusion hf polymerase new england biolabs m0530s ampurexp beads beckman coulter a63882 zeocin invitrogen r25005  (Thermo Fisher)
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Thermo Fisher recombinant proteins phusion hf polymerase new england biolabs m0530s ampurexp beads beckman coulter a63882 zeocin invitrogen r25005
Infrared gel electrophoresis images of DNase I footprinting products following incubation of compounds 1–6 with <t>TOPOIIα</t> promoter DNA. Compounds were incubated for 17 h with TOPOIIα promoter DNA IR-labelled on the sense (A) or antisense (B) strand. Each gel consists of 50 lanes: two GA marker lanes (GA) and six sets of eight lanes for compound (l–r; 0, 0.0016, 0.008, 0.04, 0.2, 1, 5 and 25 μM). The sequence positions relating to Supplementary Figure 1 are given vertically.
Recombinant Proteins Phusion Hf Polymerase New England Biolabs M0530s Ampurexp Beads Beckman Coulter A63882 Zeocin Invitrogen R25005, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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recombinant proteins phusion hf polymerase new england biolabs m0530s ampurexp beads beckman coulter a63882 zeocin invitrogen r25005 - by Bioz Stars, 2026-10
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gene exp ssbp1 hs00995378 m1  (Thermo Fisher)
86
Thermo Fisher gene exp ssbp1 hs00995378 m1
Infrared gel electrophoresis images of DNase I footprinting products following incubation of compounds 1–6 with <t>TOPOIIα</t> promoter DNA. Compounds were incubated for 17 h with TOPOIIα promoter DNA IR-labelled on the sense (A) or antisense (B) strand. Each gel consists of 50 lanes: two GA marker lanes (GA) and six sets of eight lanes for compound (l–r; 0, 0.0016, 0.008, 0.04, 0.2, 1, 5 and 25 μM). The sequence positions relating to Supplementary Figure 1 are given vertically.
Gene Exp Ssbp1 Hs00995378 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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recombinant adenovirus containing cdna of dominant-negative pkc ad-pkc k281r  (Johns Hopkins HealthCare)
90
Johns Hopkins HealthCare recombinant adenovirus containing cdna of dominant-negative pkc ad-pkc k281r
Infrared gel electrophoresis images of DNase I footprinting products following incubation of compounds 1–6 with <t>TOPOIIα</t> promoter DNA. Compounds were incubated for 17 h with TOPOIIα promoter DNA IR-labelled on the sense (A) or antisense (B) strand. Each gel consists of 50 lanes: two GA marker lanes (GA) and six sets of eight lanes for compound (l–r; 0, 0.0016, 0.008, 0.04, 0.2, 1, 5 and 25 μM). The sequence positions relating to Supplementary Figure 1 are given vertically.
Recombinant Adenovirus Containing Cdna Of Dominant Negative Pkc Ad Pkc K281r, supplied by Johns Hopkins HealthCare, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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recombinant adenovirus encoding human fviii av1h8101  (Genetic Therapy Inc)
90
Genetic Therapy Inc recombinant adenovirus encoding human fviii av1h8101
Infrared gel electrophoresis images of DNase I footprinting products following incubation of compounds 1–6 with <t>TOPOIIα</t> promoter DNA. Compounds were incubated for 17 h with TOPOIIα promoter DNA IR-labelled on the sense (A) or antisense (B) strand. Each gel consists of 50 lanes: two GA marker lanes (GA) and six sets of eight lanes for compound (l–r; 0, 0.0016, 0.008, 0.04, 0.2, 1, 5 and 25 μM). The sequence positions relating to Supplementary Figure 1 are given vertically.
Recombinant Adenovirus Encoding Human Fviii Av1h8101, supplied by Genetic Therapy Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Inhibitory effects of gemifloxacin and ciprofloxacin on wild-type and mutant ( grlA -deficient) topoisomerase IV and wild-type gyrase

Journal:

Article Title: Topoisomerase Targeting with and Resistance to Gemifloxacin in Staphylococcus aureus

doi: 10.1128/AAC.47.1.274-282.2003

Figure Lengend Snippet: Inhibitory effects of gemifloxacin and ciprofloxacin on wild-type and mutant ( grlA -deficient) topoisomerase IV and wild-type gyrase

Article Snippet: Conditions for reconstitution of S. aureus topoisomerase IV and DNA gyrase and assay conditions for inhibition of kinetoplast DNA (kDNA) (from Crithidia fasciculata ; TopoGEN Inc., Columbus, Ohio) decatenation by topoisomerase IV and DNA supercoiling by gyrase have been published previously, except that 200 ng of kDNA was used for decatenation assays ( 26 ).

Techniques: Mutagenesis

Infrared gel electrophoresis images of DNase I footprinting products following incubation of compounds 1–6 with TOPOIIα promoter DNA. Compounds were incubated for 17 h with TOPOIIα promoter DNA IR-labelled on the sense (A) or antisense (B) strand. Each gel consists of 50 lanes: two GA marker lanes (GA) and six sets of eight lanes for compound (l–r; 0, 0.0016, 0.008, 0.04, 0.2, 1, 5 and 25 μM). The sequence positions relating to Supplementary Figure 1 are given vertically.

Journal: Nucleic Acids Research

Article Title: A 96-well DNase I footprinting screen for drug–DNA interactions

doi: 10.1093/nar/gkm467

Figure Lengend Snippet: Infrared gel electrophoresis images of DNase I footprinting products following incubation of compounds 1–6 with TOPOIIα promoter DNA. Compounds were incubated for 17 h with TOPOIIα promoter DNA IR-labelled on the sense (A) or antisense (B) strand. Each gel consists of 50 lanes: two GA marker lanes (GA) and six sets of eight lanes for compound (l–r; 0, 0.0016, 0.008, 0.04, 0.2, 1, 5 and 25 μM). The sequence positions relating to Supplementary Figure 1 are given vertically.

Article Snippet: The proximal 705 bp of the human Topoisomerase II alpha (TOPOIIα) promoter was amplified by PCR from human genomic DNA (Promega) using primers TIIA-F (CACCGCACACAGCCTAC) and TIIA-R (TGGTGACGGTCGTGAAG) (Supplementary Figure 1).

Techniques: Nucleic Acid Electrophoresis, Footprinting, Incubation, Marker, Sequencing

A comparison of footprint profiles of compounds 1–6 on the proximal region of the TOPOIIα promoter. Single footprint profiles were program-generated starting with the IR footprint gel image shown in A. Where no footprinting is seen, the sequence has been given an arbitrary score of −3.9 (log 125 μM). The DNA sequence is given in Supplementary Figure 1.

Journal: Nucleic Acids Research

Article Title: A 96-well DNase I footprinting screen for drug–DNA interactions

doi: 10.1093/nar/gkm467

Figure Lengend Snippet: A comparison of footprint profiles of compounds 1–6 on the proximal region of the TOPOIIα promoter. Single footprint profiles were program-generated starting with the IR footprint gel image shown in A. Where no footprinting is seen, the sequence has been given an arbitrary score of −3.9 (log 125 μM). The DNA sequence is given in Supplementary Figure 1.

Article Snippet: The proximal 705 bp of the human Topoisomerase II alpha (TOPOIIα) promoter was amplified by PCR from human genomic DNA (Promega) using primers TIIA-F (CACCGCACACAGCCTAC) and TIIA-R (TGGTGACGGTCGTGAAG) (Supplementary Figure 1).

Techniques: Generated, Footprinting, Sequencing